Evaluating the Efficacy of Bioaugmentation for In-Situ Treatment of PCB Impacted Sediments

ER-201215

Objective

This project aims to reduce the concentration of polychlorinated biphenyls (PCBs) in sediments by the in-situ application of a "biocatalytic" activated carbon amendment seeded with PCB degrading microorganisms. Introduction of PCB dechlorinating and degrading microorganisms as biofilms on SediMite™, an innovative solid substrate that includes activated carbon particles, will achieve two major objectives: (1) immediate reduction of PCB bioavailability to the aquatic food chain through sequestration on the carbon surface, and (2) reduction of in situ PCB concentrations in impacted sediments by microbial dechlorination and subsequent aerobic biodegradation of the PCBs. A pilot study will be carried out to demonstrate and validate this environmentally sustainable technology at a PCB-impacted Department of Defense (DoD) field site. The project will evaluate the efficacy of bioaugmentation for the complete degradation of highly chlorinated Aroclors in situ and of a delivery system for dispersing biocatalysts into PCB-impacted sediment through a water column in environmentally sensitive areas.

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Technology Description

Although the feasibility of bioaugmentation for PCB dechlorination has been demonstrated in the laboratory, an effective delivery system to introduce microorganisms into sediments has not been available. Recent work supported by SERDP and EPA's Small Business Innovation Research Program has led to the development of a pelletized delivery system, SediMite, for introducing sorbent amendments into sediments. Biocatalaytic SediMite is applied as a solid substrate for delivery of microorganisms into sediments, formation of microbial biofilms, and sequestration and concentration of hydrophobic PCBs in close proximity to the biofilm of PCB transforming bacteria. Recent laboratory mesocosm-scale experiments have demonstrated the long-term activity of the microorganisms delivered with SediMite and the overall feasibility of the approach. This ESTCP project will include production-level scale-up of the microorganisms, production of modified SediMite containing electron donors and nutrients, development of a nozzle system to introduce active PCB transforming microorganisms into SediMite pellets during dispersal of the pellets at the site, a treatability study for site sediments, and a pilot application at a DoD facility to test field effectiveness.

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Benefits

DoD facilities across the country are impacted with persistent organic pollutants (POPs) such as PCBs, and remediation of these sites is challenging. This work addresses the need for cost-effective, in situ remediation technologies for PCBs that can in principle be applied to other POPs such as pesticides. Most importantly, this work will enable extensive in situ treatment at DoD sites that include both shallow and deep sediments with minimum impact to environmentally sensitive areas. The integrated approach utilizes activated carbon to serve concurrently as an agent to sequester PCBs from the food chain and as a delivery system and solid substrate to enhance both anaerobic and aerobic microbial processes for complete in situ degradation. Development of a tractable microbial in situ treatment system would provide a practical, cost-effective, and environmentally sustainable means of treating POPs. The technology is expected to cost approximately $110,000 per acre, which compares favorably with capping (approximately $115,000 to 290,000 per acre) and dredging (approximately $1 million per acre). In terms of the life-cycle assessment, as compared to dredging, use of biocatalytic SediMite would reduce the health risks associated with sediment disruption, reduce overall energy use, effectively negate the requirement for extensive waste management, and obviate the requirement for substantial habitat restoration. (Anticipated Project Completion - 2015)

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Points of Contact

Principal Investigator

Dr. Kevin Sowers

University of Maryland

Phone: 410-234-8878

Fax: 410-468-3912

Program Manager

Environmental Restoration

SERDP and ESTCP

Document Types

  • Fact Sheet - Brief project summary with links to related documents and points of contact.
  • Final Report - Comprehensive report for every completed SERDP and ESTCP project that contains all technical results.
  • Cost & Performance Report - Overview of ESTCP demonstration activities, results, and conclusions, standardized to facilitate implementation decisions.
  • Technical Report - Additional interim reports, laboratory reports, demonstration reports, and technology survey reports.
  • Guidance - Instructional information on technical topics such as protocols and user’s guides.
  • Workshop Report - Summary of workshop discussion and findings.
  • Multimedia - On demand videos, animations, and webcasts highlighting featured initiatives or technologies.
  • Model/Software - Computer programs and applications available for download.
  • Database - Digitally organized collection of data available to search and access.